Mouse Model for Generation of Antibodies and Vaccine Antigens
Inventors: Frederick Alt, Hwei-Ling Cheng, Ming Tian
Invention Types: Therapeutics, Research Tool
Research Areas: Immunology
Keywords: Antibody, Vaccine, Mouse Model, PlatformFor More Information Contact: Ives, Catherine L.
Improved methods of producing variants of existing antibodies with higher affinities or modified specificities are important for clinical applications. Researchers at Boston Children’s Hospital have developed an antibody development platform utilizing a mouse model with a novel engineered immune system for the development of such antibody variants. The system allows rapid and efficient expression of any desired combination human variable region (VH, D, J, DJ, or VL) gene segments in mice to derive novel variant antibodies with improved affinity and/or specificity of known human antibodies including therapeutically useful antibodies.
• Method to improve affinity and/or specificity of any therapeutic antibody for clinical application.
• Method to evaluate the efficacy of vaccine candidates and immunization protocols on affinity maturation, such as protection from HIV infection by Broadly Neutralizing Antibodies (BnAbs).
• In less than 9 months, researchers can generate a cohort of mice for immunization that express any desired human variable region exons in a large fraction of peripheral B cells.
• Model should be applicable for improving affinity/specificity of many antibodies generated by current immunization and phage display methods.
• Relatively inexpensive model to test vaccine approaches for the development of unusual (e.g. highly mutated) antibody responses (e.g. certain HIV broadly neutralizing antibodies).
• Platform for sponsored research
• Startup or license opportunity
Key Publications: Lin SG, Ba Z, Du Z, et al. Highly sensitive and unbiased approach for elucidating antibody repertoires. PNAS 2016 Jul 12;113(28).
IPStatus: Pat. Pend.