Chemical inducer of dimerization cleavable by light
Inventors: Thomas Schwarz, PhD, Matthew Banghart, Amos Gutnick
Invention Types: Research Tool
Keywords: Cell SignalingFor More Information Contact: Ives, Catherine L.
Researchers in the lab of Dr. Schwarz have developed a method to control the function of proteins within a cell by using a dimerizing agent that is cleaved by light. Chemical inducers of dimerization are composed of either two identical ligands joined to one another (homo-dimerizing) or two different ligands joined to one another (heterodimerizers). The researchers' strategy is to insert a third element between the two ligands that can be cleaved by light to split the two ligand groups apart. The researchers developed a photo-cleavable chemical inducer of dimerization that consists of two different protein-binding ligands connected by a linker that can be broken by exposure to 405nm light. This compound allows the researcher to gain control of engineered proteins within cells by adding the compound and subsequently inactivating it at will with light. This allows the experimenter to control precisely when and where in the cell the link is broken.
A significant new research tool for the analysis of protein function and pathways.
• Turning off or removing a protein from a cell is a common strategy for probing the function of a protein or process.
• Similarly, turning on a protein at will can be a valuable tool for studying its consequences.
• Compound can be used for either of these purposes and potentially for other forms of regulation of a protein inside a cell.
• This is the only chemical inducers of dimerization that has been engineered to be cleaved by light, the crucial advantage of our compound.
IPStatus: Pat. Pend.