Monoclonal Antibodies to Native Murine CCR9
Inventors: James Campbell, Marc-Andrae Wurbel
Invention Types: Research Tool
Research Areas: Gastrointestinal/Nutrition, Immunology
Keywords: Antibody, ReagentFor More Information Contact: Ives, Catherine L.
The CCR9 receptor is a G protein-coupled seven-transmembrane receptor expressed on thymocytes and other intestine-homing cells. It has been proposed that CCR9 is necessary to allow lymphocytes access to intestinal sites. Drugs that block CCR9 from binding with its chemokine ligand CCL25 or TECK may be useful treatments for inflammatory bowel diseases. Six monoclonal antibodies that detect murine CCR9 have been generated.
CCR9-deficient mice were immunized with murine thymocytes expressing native CCR9. B cells from the immunized mice were fused to a murine myeloma to produce six hybridoma clones. No cross-reactivity with human and rat CCR9 has been identified. The antibodies have been tested for use in flow cytometry and immunohistochemistry.
Since these anti-mouse antibodies are of mouse origin, they will be tolerated upon injection into mice and may be helpful in animal models to assess the usefulness of potential drugs that block CCR9 function.
Key Publications: Wurbel el al (2006). Complex Regulation of CCR9 at Multiple Discrete Stages of T Cell Development. European Journal of Immunology 36, 73-81.
IPStatus: Not applicable