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CMCC 3161

Method to derive microglia from hematopoietic progenitor and stem cells

Inventors: Bruna Paulsen, Derrick Rossi, Manavi Nagai

Invention Types: Research Tool

Research Areas: Neurology/Neuroscience

Keywords: Assay, Cell Line, Cell Therapy, CNS, Drug Screening

For More Information Contact:  Dietz, Ryan

 

Invention Description:

As the resident immune cells of the central nervous system, microglia play crucial roles in surveillance, pruning/neuromodulation and inflammation, among other functions in both the healthy and diseased brain. Current protocols to study microglia in vitro depend on their isolation from post mortem brain tissue – a limited source. Methods exist to differentiate microglia from peripheral blood cells, monocytes, and embryonic stem cells; but, these methods are limited by the number of cells, time, and labor required.

Derrick Rossi, PhD, and colleagues have developed a method to circumvent these limitations. Using a proprietary expansion media and protocol, the group is able to isolate rare hematopoietic progenitors and stem cells from peripheral blood and effectively expand to sufficient numbers for differentiation. These cells are then successfully differentiated into microglia-like cells, as determined by their morphology, cytokine profile, and ability to phagocytose foreign materials.

Applications:

• Platform to study interventions or characteristics of microglia in different disease states
• Platform to perform screens for therapeutics targets and drugs to treat microglial dysfunction
• Possibility of expanding hematopoietic stem cell-derived microglia to be used in transplantation as therapy for neurological disorders

Competitive Advantages:

• Robust clonal expansion from just one hematopoietic stem cell or CD34+ cell
• Maintenance of stem-ness through expansion using proprietary media formulation

Business Opportunity:

Non-exclusive or exclusive license

IPStatus: Pat. Pend.