GlycoPipe: Simple and Sensitive Glycoproteomics
Inventors: Richard Lee, John Froehlich
Invention Types: Diagnostic/Prognostic, Information Technology/Software
Research Areas: Personalized Medicine, Pathology
Keywords: Drug Discovery, Instrumentation, Protein, ProteomicsFor More Information Contact: Khunkhun, Rajinder
Glycoproteins are proteins with a glycosylation modification, and comprise over 50% of the human proteome. Glycans are a group of carbohydrate side chains covalently linked to the asparagine residues (N-linked) and serine or threonine residues (O-linked) of proteins, which are involved in numerous biological functions such as cell-cell signaling, structural modulation of glycoproteins, and pathogen-host recognition.
Changes in glycosylation have long been known to be associated with a wide range of diseases, including the genetic congenital disorders of glycosylation (CDG), diabetes, viral infection, autoimmune disorders, and tumor progression. Furthermore, consistent glycosylation is a critical aspect of quality control in the production of therapeutic glycoproteins, because consistent glycosylation significantly affects the overall efficiency, efficacy, and safety of therapeutic glycoproteins. Therefore, the development of a mass spectrometry (MS)-based strategy to measure quantitative changes of protein glycosylation is desired for academic, biotechnological, and pharmaceutical laboratories.
There is a significant need for novel, user-friendly software to allow for accurate detection and quantitation of intact glycoproteins. John Froehlich and Richard Lee have developed a software specifically designed for the non-expert common user who may be studying a protein that is glycosylated. Applications range from fundamental biochemistry to precision medicine drug design.
• Precision medicine: empower analysis in varied fields which are known to be influenced by glycosylation
• Drug design: variations in the glycosylation of many biologics are the critical driver in drug novelty, efficacy and safety
• Ease of use – No need to manually annotate spectra (meaning extensive application-specific training not required)
• Improved sensitivity over industry standard
• Interrogates intact glycopeptides, not requiring preexisting knowledge of glycoprotein of interest
IPStatus: Pat. in Prep.